Assay of hemoglobin A1c using lectin from Aleuria aurantia
نویسندگان
چکیده
منابع مشابه
Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL
Protein-carbohydrate interactions are very often mediated by the stacking CH-π interactions involving the side chains of aromatic amino acids such as tryptophan (Trp), tyrosine (Tyr) or phenylalanine (Phe). Especially suitable for stacking is the Trp residue. Analysis of the PDB database shows Trp stacking for 265 carbohydrate or carbohydrate like ligands in 5 208 Trp containing motives. An app...
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We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using...
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The carbohydrate-binding specificity of Aleuria aurantia lectin was investigated by analyzing the behavior of a variety of fucose-containing oligosaccharides on an A. aurantia lectin-Sepharose column. Studies with complex-type oligosaccharides obtained from various glycoproteins by hydrazinolysis and their partial degradation fragments indicated that the presence of the alpha-fucosyl residue li...
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Two very interesting articles on hemoglobin A1c (Hb A1c) 2 method performance appear in this issue of Clinical Chemistry. Woodworth et al. (1 ) use Hb A1c as an example of how the risk of reporting inaccurate results can be estimated to guide individualized QC strategies. They assess the risk of reporting unreliable Hb A1c results with 6 different methods in 4 academic medical centers; 5 are la...
متن کاملAn automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c.
An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contai...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2016
ISSN: 2191-0855
DOI: 10.1186/s13568-016-0288-7